Effects of dissolved oxygen concentration and iron addition on immediate-early gene expression of Magnetospirillum gryphiswaldense MSR-1
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چکیده
منابع مشابه
Effects of Environmental Conditions on High-Yield Magnetosome Production by Magnetospirillum gryphiswaldense MSR-1
Background: Magnetotactic bacteria are a heterogeneous group of Gram-negative prokaryote cells that produce linear chains of magnetic particles called magnetosomes, intracellular organelles composed of magnetic iron particles. Many important applications have been defined for magnetic nanoparticles in biotechnology, such as cell separation applications and acting as carriers of enzymes, antib...
متن کاملExpression patterns of key iron and oxygen metabolism genes during magnetosome formation in Magnetospirillum gryphiswaldense MSR-1.
To evaluate the expression patterns of genes involved in iron and oxygen metabolism during magnetosome formation, the profiles of 13 key genes in Magnetospirillum gryphiswaldense MSR-1 cells cultured under high-iron vs. low-iron conditions were examined. Cell growth rates did not differ between the two conditions. Only the high-iron cells produced magnetosomes. Transmission electron microscopy ...
متن کاملComplete Genome Sequence of Magnetospirillum gryphiswaldense MSR-1
We report the complete genomic sequence of Magnetospirillum gryphiswaldense MSR-1 (DSM 6361), a type strain of the genus Magnetospirillum belonging to the Alphaproteobacteria. Compared to the reported draft sequence, extensive rearrangements and differences were found, indicating high genomic flexibility and "domestication" by accelerated evolution of the strain upon repeated passaging.
متن کاملIdentification of promoters for efficient gene expression in Magnetospirillum gryphiswaldense.
To develop an expression system for the magnetotactic bacterium Magnetospirillum gryphiswaldense, we compared gene expression from the widely used Escherichia coli P(lac) promoter with that from known and predicted genuine M. gryphiswaldense promoters. With the use of green fluorescent protein as a reporter, the highest expression level was observed with the magnetosomal P(mamDC) promoter. We d...
متن کاملEffect of Magnetospirillum gryphiswaldense on serum iron levels in mice
BACKGROUND AND OBJECTIVES The Magnetotactic bacterium Magnetospirillumgryphiswaldense (MSR-1) mineralizes the magnetite (Fe(3) O(4)) crystals and organizes a highly ordered intracellular structure, called the magnetosome. Iron transport system supports the biogenesis of magnetite. Although iron is an essential trace element for many metabolic pathways of the body, increase or decrease in iron w...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2017
ISSN: 1574-6968
DOI: 10.1093/femsle/fnx079